HPLC

Q: How is HPLC different from GC?

HPLC is suitable for non-volatile and thermally sensitive compounds , while GC is used for volatile substances.

What Is HPLC?

HPLC separates compounds based on their interaction with a liquid mobile phase and a stationary phase packed inside a column. As components elute at different retention times, they are detected and quantified using appropriate detectors.

Because HPLC operates at ambient or moderate temperatures, it is ideal for compounds that cannot be analyzed by gas chromatography.

What HPLC Measures

HPLC can be used to analyze:

Non-volatile organic compounds
Additives, stabilizers, antioxidants
Plasticizers and surfactants
Active ingredients and intermediates
Degradation products
Polar or thermally unstable compounds

Quantitative analysis is performed using calibration standards when required.

Why Use HPLC?

HPLC is chosen when:

target compounds are non-volatile or heat-sensitive
precise quantification is required
complex mixtures need reliable separation
formulation or impurity profiling is needed

Typical questions HPLC can answer:

What additives are present and at what levels?
Are impurities or degradation products present?
Do two materials have the same chemical composition?
Has processing altered the formulation?
Are batches consistent with specifications?

Typical Application Scenarios

Impurity & Degradation Analysis

Detection and quantification of impurities
Stability and aging studies
Investigation of unexpected performance changes

Deformulation & Reverse Engineering

Identification of formulation components
Comparison of competitor products
Support for formulation optimization

Polymers, Coatings & Adhesives

Analysis of additives and stabilizers
Evaluation of chemical changes after processing
Comparison of “good vs. failed” samples

Pharmaceuticals & Medical Products

Active ingredient and impurity analysis
Stability and compatibility studies
Quality control and validation support

Quality Control & Supplier Qualification

Batch-to-batch consistency verification
Incoming material inspection
Process change validation

Sample Types

HPLC can be applied to:

liquids and solutions
solids after appropriate extraction
polymers, resins, and coatings
adhesives, sealants, and consumer products

Sample preparation may include solvent extraction, dilution, filtration, or derivatization, depending on the analytical objective.

What You Will Receive

Each HPLC project is delivered with a clear, structured report designed for technical and business decision-making. A typical deliverable includes:

test objective and sample description
sample preparation and HPLC conditions
chromatograms with peak identification
qualitative and/or quantitative results
comparison summaries (batch vs. batch, supplier vs. supplier)
interpretation of findings and potential impact
recommendations for follow-up testing or corrective actions

Why Choose Xinbodi for HPLC?

Experience with complex chemical and material matrices
Flexible method development tailored to your application
Accurate and reproducible quantitative results
Clear interpretation beyond chromatograms
Support for R&D, QC, deformulation, and failure investigations
Confidential handling of proprietary formulations and data