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UVVIS/NIR

What is UV-Vis/NIR Spectroscopy?

UV-Vis/NIR spectroscopy measures how a sample absorbs or transmits light across the ultraviolet (UV), visible (Vis), and near-infrared (NIR) regions. The resulting spectrum reveals information related to electronic transitions (UV-Vis) and overtone/combination vibrational bands (NIR), supporting both quantitative concentration measurement and material/optical property characterization.

UV-Vis/NIR is widely used for solutions, thin films, coatings, glasses, polymers, semiconductors, nanoparticles, and optical materials for applications such as color control, absorbance/transmittance, band-edge behavior, and process/QC monitoring.

What UV-Vis/NIR Can Help You Solve

  • Concentration/assay measurement using Beer–Lambert law (with standards or calibration curve)

  • Color and appearance control (absorbance spectrum, color consistency checks)

  • Optical performance evaluation (transmittance/absorbance in UV/Vis/NIR ranges)

  • Material comparison (batch-to-batch, supplier comparison, before/after aging)

  • Thin film & coating characterization (absorbance/transmittance vs wavelength; comparative QC)

  • Nanomaterials screening (plasmon peaks, dispersion quality—project-dependent)

Typical Applications

  • Chemicals & solutions: concentration verification, impurity screening by spectral changes

  • Pharmaceutical & biotech: protein/nucleic acid absorbance (project-dependent), assay support

  • Optical materials: glass, filters, lenses, films—UV cut-off and NIR transmission checks

  • Coatings & polymers: UV-blocking coatings, optical clarity, aging/yellowing evaluation

  • Semiconductor & displays: substrate/film transmission, absorption edge trends (project-dependent)

  • Nanoparticles & pigments: dispersion and peak shift monitoring (project-dependent)

Test Capabilities & What You Receive

Measurement Modes (project-dependent)

  • Absorbance / Transmittance / Reflectance vs wavelength

  • Solution testing (cuvette-based)

  • Solid and film testing (transmission or reflectance configurations, as applicable)

  • Baseline correction and comparative overlays for multiple samples

  • Quantitative analysis (single-point using known absorptivity or calibration curve)

Deliverables

  • UV-Vis/NIR spectra (plots and data)

  • Key metrics at specified wavelengths (e.g., %T at 550 nm, UV cut-off wavelength)

  • For quantitative projects: calculated concentration with method details and QC checks

  • Comparison summary and pass/fail vs your specification (if provided)

Sample Requirements

Liquids / Solutions

  • Typical volume: ~2–5 mL (depends on cuvette type and repeats)

  • Clarity: minimize bubbles and particulates (filter if compatible)

  • Solvent/matrix: provide solvent identity and expected concentration range

  • Containers: clean, tightly sealed, labeled

Solids / Films

  • Flat, clean surfaces preferred; provide thickness if known

  • Indicate whether measurement is transmission (transparent samples) or reflectance (opaque samples)

Information to provide: target wavelength range (UV/Vis/NIR), required reporting metrics, spec limits, and any reference/control sample.

Workflow

  • Requirement review (measurement mode, wavelength range, quant vs comparison, spec limits)

  • Sample preparation guidance (dilution, filtration, cuvette/path length selection, mounting for solids)

  • Instrument setup (baseline/reference, scan parameters)

  • Measurement and repeat checks as needed

  • Data processing (baseline correction, peak/edge metrics, calibration calculations)

  • Reporting (spectra + results table + conclusions)

FAQs

UV-Vis/NIR is great for comparison and for systems with known spectral signatures, but it is not always definitive for unknown identification. For unknowns, complementary methods such as FTIR, Raman, GC/MS, or LC-MS may be recommended.

Yes—typically by dilution, using a shorter path length, or adjusting measurement configuration. Provide expected absorbance range if known.

UV-Vis mainly reflects electronic transitions (color, band-edge behavior). NIR often relates to overtone/combination bands and can be useful for certain compositional or moisture-related trends (matrix-dependent).

Yes. Tell us your required wavelengths and acceptance criteria, and we will report the specified metrics.

Yes. If you provide standards (or we prepare them per your guidance), we can build a calibration curve and report concentration with QC checks.

Have additional questions?
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  • yangxbd@gmail.com